Antibodies from patients with Parkinson's disease react with protein modified by dopamine oxidation
Identifieur interne : 001F23 ( Main/Exploration ); précédent : 001F22; suivant : 001F24Antibodies from patients with Parkinson's disease react with protein modified by dopamine oxidation
Auteurs : Dominic B. Rowe [États-Unis] ; Weidong Le [États-Unis] ; R. Glenn Smith [États-Unis] ; Stanley H. Appel [États-Unis]Source :
- Journal of Neuroscience Research [ 0360-4012 ] ; 1998-09-01.
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Abstract
To determine whether specific antibodies are present in PD, we used an enzyme‐linked immunosorbant assay (ELISA) that identifies increased immunoglobulin (IgG) levels towards a synthetic substrate prepared by incubating ovalbumin with dopamine and copper sulfate. Altered absorption spectrum and specific chemical detection demonstrated quinone modification of the ovalbumin. This modified protein was demonstrated to react with serial dilutions of PD sera. A threshold dilution of 1:500 was subsequently used to screen sera from patients with PD (n = 21), amyotrophic lateral sclerosis (n = 7), Alzheimer's disease (n = 7) and other neurological disease controls (n = 7). The assay produced a positive result in 7/21 PD patients and 0/21 disease controls (P < 0.02, Kruskal–Wallis test). Further testing of sera from untreated PD patients (n = 6) identified one positive sample. Thus, a subset of Parkinson's disease (PD) patients has immunoglobulin (IgG) to ovalbumin modified by dopamine oxidation. The presence of antibody reactivity to quinone‐modified proteins could contribute to or amplify the inflammatory response in PD. J. Neurosci. Res. 53:551–558, 1998. © 1998 Wiley‐Liss, Inc.
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DOI: 10.1002/(SICI)1097-4547(19980901)53:5<551::AID-JNR5>3.0.CO;2-8
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Rowe</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Texas</region></placeName><wicri:cityArea>Department of Neurology, Baylor College of Medicine, Houston</wicri:cityArea></affiliation></author><author><name sortKey="Le, Weidong" sort="Le, Weidong" uniqKey="Le W" first="Weidong" last="Le">Weidong Le</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Texas</region></placeName><wicri:cityArea>Department of Neurology, Baylor College of Medicine, Houston</wicri:cityArea></affiliation></author><author><name sortKey="Smith, R Glenn" sort="Smith, R Glenn" uniqKey="Smith R" first="R. Glenn" last="Smith">R. 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Res.</title><idno type="ISSN">0360-4012</idno><idno type="eISSN">1097-4547</idno><imprint><publisher>John Wiley & Sons, Inc.</publisher><pubPlace>New York</pubPlace><date type="published" when="1998-09-01">1998-09-01</date><biblScope unit="volume">53</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="551">551</biblScope><biblScope unit="page" to="558">558</biblScope></imprint><idno type="ISSN">0360-4012</idno></series><idno type="istex">D295E788DA5A040304BC10DD7F5419B9E7707D73</idno><idno type="DOI">10.1002/(SICI)1097-4547(19980901)53:5<551::AID-JNR5>3.0.CO;2-8</idno><idno type="ArticleID">JNR5</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0360-4012</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>dopamine</term><term>immunity</term><term>immunoglobulin</term><term>quinone</term><term>quinoprotein</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To determine whether specific antibodies are present in PD, we used an enzyme‐linked immunosorbant assay (ELISA) that identifies increased immunoglobulin (IgG) levels towards a synthetic substrate prepared by incubating ovalbumin with dopamine and copper sulfate. Altered absorption spectrum and specific chemical detection demonstrated quinone modification of the ovalbumin. This modified protein was demonstrated to react with serial dilutions of PD sera. A threshold dilution of 1:500 was subsequently used to screen sera from patients with PD (n = 21), amyotrophic lateral sclerosis (n = 7), Alzheimer's disease (n = 7) and other neurological disease controls (n = 7). The assay produced a positive result in 7/21 PD patients and 0/21 disease controls (P < 0.02, Kruskal–Wallis test). Further testing of sera from untreated PD patients (n = 6) identified one positive sample. Thus, a subset of Parkinson's disease (PD) patients has immunoglobulin (IgG) to ovalbumin modified by dopamine oxidation. The presence of antibody reactivity to quinone‐modified proteins could contribute to or amplify the inflammatory response in PD. J. Neurosci. Res. 53:551–558, 1998. © 1998 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Texas</li></region></list><tree><country name="États-Unis"><region name="Texas"><name sortKey="Rowe, Dominic B" sort="Rowe, Dominic B" uniqKey="Rowe D" first="Dominic B." last="Rowe">Dominic B. Rowe</name></region><name sortKey="Appel, Stanley H" sort="Appel, Stanley H" uniqKey="Appel S" first="Stanley H." last="Appel">Stanley H. Appel</name><name sortKey="Le, Weidong" sort="Le, Weidong" uniqKey="Le W" first="Weidong" last="Le">Weidong Le</name><name sortKey="Smith, R Glenn" sort="Smith, R Glenn" uniqKey="Smith R" first="R. Glenn" last="Smith">R. Glenn Smith</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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